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If a PC sends 24bit pixels to a MAC, the 32bit word must have the reserved byte as zero so the MAC can determine what 8 bits is blue, then swap.
'must be zero' would not apply to 32 bit alpha image, and really only apply in an windows function that explicitly takes a RGBQuad as a parameter, and even then, 'must be zero' I think was auto inserted any time a developer left a parameter undocumented , as in the case of future media types.
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